• Optical nanoscopy of tissue sections 

      Villegas Hernandez, Luis Enrique (Master thesis; Mastergradsoppgave, 2018-05-28)
      The study of organ tissues, namely, histology, is essential for clinical medicine, as it allows the identification of a wide range of diseases. Traditionally, two major techniques, optical and electron microscopy, have been used for morphological observation of tissue sections. Optical microscopy (OM) allows for fast-acquisition high-throughput imaging, at the expense of diffraction-limited resolution ...
    • Visualizing ultrastructural details of placental tissue with super-resolution structured illumination microscopy 

      Villegas Hernandez, Luis Enrique; Nystad, Mona; Ströhl, Florian; Basnet, Purusotam; Acharya, Ganesh; Ahluwalia, Balpreet Singh (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-14)
      Super-resolution fluorescence microscopy is a widely employed technique in cell biology research, yet remains relatively unexplored in the field of histopathology. Here, we describe the sample preparation steps and acquisition parameters necessary to obtain fluorescent multicolor super-resolution structured illumination microscopy (SIM) images of both formalin-fixed paraffin-embedded and cryo-preserved ...